Anti-CtIP (phospho S738) antibody [EP18893] (ab195291)
Key features and details
- Rabbit monoclonal [EP18893] to CtIP (phospho S738)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CtIP (phospho S738) antibody [EP18893]
See all CtIP primary antibodies -
Description
Rabbit monoclonal [EP18893] to CtIP (phospho S738) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: HEK-293 untreated, and treated with 60 J/m² UV light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP18893 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CtIP (phospho S738) antibody [EP18893] (ab195291) at 1/1000 dilution
Lane 1 : Untreated HEK-293 (human epithelial cell line from embryonic kidney) cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate
Lane 2 : HEK-293 treated with 60 J/m² UV light, then cultivated in RPMI 1640 media containing 10% FBS for 2 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of CtIP (phospho S738) labeled with ab195291 at 1/1000 dilution.
Lane 1: CtIP (phospho S738) peptide;
Lane 2: CtIP non-phospho peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution was used as secondary antibody.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.