Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

Key features and details

  • Mouse monoclonal [OTI4D8] to CTAG1B - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free
    See all CTAG1B primary antibodies

  • Description

    Mouse monoclonal [OTI4D8] to CTAG1B - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant full length protein corresponding to Human CTAG1B. Produced in HEK-293T cells.
    Database link: P78358

  • Positive control

    • WB: pCMV6-ENTRY CTAG1B transfected HEK-293T cell lysate. A-375 extracts. IHC-P: Human pancreas and testis tissue. ICC: pCMV6-ENTRY CTAG1B transfected COS-7 cells. Flow Cyt: Living HEK-293T cells transfected with CTAG1B overexpression plasmid. NCI-H1299 and A-375 cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Constituents: 92% PBS, 8% Trehalose

    Lyophilized from.

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    OTI4D8

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    All lanes : Mouse monoclonal [OTI4D8] to CTAG1B at 1/2000 dilution

    Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY CTAG1B cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 18 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Transfected for 48 hrs then lysed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Paraffin embedded human pancreas tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3mins.

     

  • Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Immunocytochemistry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY CTAG1B stained for CTAG1B (green) using a mouse monoclonal [OTI4D8] to CTAG1B at 1/100 dilution in ICC.

  • Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Western blot - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    All lanes : Mouse monoclonal [OTI4D8] to CTAG1B at 1/500 dilution

    Lane 1 : NCI-H1299 (Human lung carcinoma cell line) cell extracts
    Lane 2 : A-375 (Human malignant melanoma cell line) cell extracts

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 18 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Paraffin embedded human testis tissue stained for CTAG1B using a mouse monoclonal [OTI4D8] to CTAG1B at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer, pH9.0, 120°C for 3mins.

     

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with CTAG1B overexpression plasmid (Red) and empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control at 1/100 dilution.

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living NCI-H1299 (Human lung carcinoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

  • Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)
    Flow Cytometry - Anti-CTAG1B antibody [OTI4D8] - BSA and Azide free (ab275373)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

    Flow cytometric analysis of living A-375 (Human malignant melanoma cell line) cells, using anti-CTAG1B antibody (Red), compared to an isotype control (green) at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.