Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)
![Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-410612-anti-saa1-saa2-antibody-epr19550-bsa-and-azide-free-western-blot.jpg "Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19550] to SAA1 + SAA2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free
See all SAA1 + SAA2 primary antibodies -
Description
Rabbit monoclonal [EPR19550] to SAA1 + SAA2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251476 is the carrier-free version of ab207445. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251476 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19550 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)All lanes : Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution
Lane 1 : Human SAA1 active recombinant protein
Lane 2 : Human SAA2 fragment recombinant protein
Lane 3 : Human SAA4 fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 13,35 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human SAA2 and SAA4 fragment recombinant protein contain aa19-122 and aa19-130 with His-Tag® and GST-tag.
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![Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-408304-anti-saa1-saa2-antibody-epr19550-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/5000 dilution + Human serum at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).
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![Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-406140-anti-saa1-saa2-antibody-epr19550-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)Anti-SAA1 + SAA2 antibody [EPR19550] (ab207445) at 1/1000 dilution + Human liver lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 3 minutesThis data was developed using ab207445, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Expressed by the liver; secreted in plasma and serum (PMID: 3183061; 16039662).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-2-anti-saa1-saa2-antibody-epr19550-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)This data was developed using ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-1-anti-saa1-saa2-antibody-epr19550-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)This data was developed using ab207445, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling SAA1+SAA2 with ab207445 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human liver cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)](https://www.abcam.com/ps/products/251/ab251476/Images/ab251476-6-benefits-of-recombinant-antibodies.png)
Anti-SAA1 + SAA2 antibody [EPR19550] - BSA and Azide free (ab251476)
