Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)
![Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)](https://www.abcam.com/ps/products/251/ab251119/Images/ab251119-4-anti-crnkl1-antibody-epr16064-bsa-and-azide-free-immunocytochemistry.jpg "Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16064] to CRNKL1 - BSA and Azide free
- Suitable for: ICC, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free
See all CRNKL1 primary antibodies -
Description
Rabbit monoclonal [EPR16064] to CRNKL1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251119 is the carrier-free version of ab192248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251119 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR16064 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)This data was developed using ab192248, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling CRNKL1 with ab192248 at 1/50 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody (ab150078) at 1/500 dilution. Nuclear counter stain Dapi (blue).The two negative controls are ab192248 at 1/50 dilution followed by (Goat anti mouse IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution.
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![Western blot - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)](https://www.abcam.com/ps/products/251/ab251119/Images/ab251119-405578-anti-crnkl1-antibody-epr16064-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)Anti-CRNKL1 antibody [EPR16064] (ab192248) at 1/20000 dilution + Human seminoma lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 100 kDaThis data was developed using ab192248, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)](https://www.abcam.com/ps/products/251/ab251119/Images/ab251119-2-anti-crnkl1-antibody-epr16064-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)This data was developed using ab192248, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)](https://www.abcam.com/ps/products/251/ab251119/Images/ab251119-1-anti-crnkl1-antibody-epr16064-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)This data was developed using ab192248, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CRNKL1 with ab192248 at 1/600 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
![Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)](https://www.abcam.com/ps/products/251/ab251119/Images/ab251119-5-benefits-of-recombinant-antibodies.png)
Anti-CRNKL1 antibody [EPR16064] - BSA and Azide free (ab251119)