Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRLS1 antibody (ab156882)

Key features and details

  • Rabbit polyclonal to CRLS1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CRLS1 antibody

  • Description

    Rabbit polyclonal to CRLS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Chicken, Pig, Chimpanzee, Zebrafish, Macaque monkey, Gorilla, Chinese hamster, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human CRLS1 aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: Q9UJA2

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Human Heart; Mouse Heart; Rat Heart; Human Skeletal Muscle; Mouse Skeletal Muscle; Human Liver; Rat Liver. It also gave a positive result in IHC-P in FFPE human heart tissue sections.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab156882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLS1 antibody (ab156882)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLS1 antibody (ab156882)

    IHC image of CRLS1 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156882, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-CRLS1 antibody (ab156882)
    Western blot - Anti-CRLS1 antibody (ab156882)
    All lanes : Anti-CRLS1 antibody (ab156882) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 6 : Human liver tissue lysate - total protein (ab29889)
    Lane 7 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156882 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References (3)

Publishing research using ab156882? Please let us know so that we can cite the reference in this datasheet.

ab156882 has been referenced in 3 publications.

  • Chen S  et al. In vitro fertilization alters phospholipid profiles in mouse placenta. J Assist Reprod Genet N/A:N/A (2019). PubMed: 30610659
  • Chao H  et al. Cardiolipin-Dependent Mitophagy Guides Outcome after Traumatic Brain Injury. J Neurosci 39:1930-1943 (2019). PubMed: 30626699
  • Yuan F  et al. Mitochondrial targeted peptides preserve mitochondrial organization and decrease reversible myocardial changes in early swine metabolic syndrome. Cardiovasc Res 114:431-442 (2018). PubMed: 29267873

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLS1 antibody (ab156882)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRLS1 antibody (ab156882)

    IHC image of CRLS1 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab156882, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-CRLS1 antibody (ab156882)
    Western blot - Anti-CRLS1 antibody (ab156882)
    All lanes : Anti-CRLS1 antibody (ab156882) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Heart (Rat) Tissue Lysate
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 6 : Human liver tissue lysate - total protein (ab29889)
    Lane 7 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156882 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

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