Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [KANI345B] to CRISPR-Cas9
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Streptococcus pyogenes

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [KANI345B]
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rat monoclonal [KANI345B] to CRISPR-Cas9

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Streptococcus pyogenes

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HEK-293 transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate. IHC-P: HEK-293T transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct. ICC: HEK-293T cells transfected with GFP-tagged Cas9 expression vector.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    KANI345B

  • Isotype

    IgG2a

Images

  • Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    All lanes : Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293) at 1/5000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
    Lane 2 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
    Lane 3 : HEK-293 transfected with CRISPR-Cas9 (G3ECR1, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
    Lane 4 : HEK-293 transfected with CRISPR-Cas9 (Q03JI6, Streptococcus thermophilus) with Myc-His tag, whole cell lysate
    Lane 5 : HEK-293 transfected with CRISPR-Cas9 (J7RUA5, Staphylococcus aureus subsp. aureus) with Myc-His tag, whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

    Immunohistochemical analysis of paraffin-embedded (Panel A) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct and (Panel B) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with empty plasmid, labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. 
    Positive staining on (A) HEK-293T transfected with a GFP-Myc-tagged CRISPR-associated endonuclease Cas9/Csn1 construct, no staining on (B) HEK-293T transfected with empty plasmid.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

  • Immunocytochemistry - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Immunocytochemistry - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T+OE-94 cells labelling CRISPR-Cas9 with ab271293 at 1/50 dilution (18.32 ug/ml), followed by ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
    Confocal image showing cytoplasmic staining in 293T cells transfected with GFP-tagged Cas9 expression vector.
    Secondary antibody only control: Secondary antibody is ab150160 Goat Anti-Rat IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (2 ug/ml).

  • Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Western blot - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    All lanes : Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293) at 1/5000 dilution

    Lane 1 : HEK-293 (human embryonic kidney epithelial cell) transfected with CRISPR-Cas9 (Q99ZW2, Streptococcus pyogenes serotype M1) with GFP-Myc tag, whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
    Negative control: No staining on the human tonsil.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
    Negative control: No staining on the mouse spleen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CRISPR-Cas9 with ab271293 at 1/200 (4.58 ug/ml) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
    Negative control: No staining on the rat spleen.

  • Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)
    Anti-CRISPR-Cas9 antibody [KANI345B] (ab271293)

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