Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19619-18] to CRISPR-Cas9 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP

Overview

  • Product name

    Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free
    See all CRISPR-Cas9 primary antibodies

  • Description

    Rabbit monoclonal [EPR19619-18] to CRISPR-Cas9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Streptococcus thermophilus

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate.

  • General notes

    Ab251382 is the carrier-free version of ab202637. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251382 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR19619-18

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected with Streptococcus Thermophilus Cas9 (pcDNA3.1(+)-Myc-His) with ab202637 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed. 

    Positive staining on the 293T cells transfected with Streptococcus Thermophilus Cas9.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202637).

  • Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)
    Immunoprecipitation - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)

    CRISPR-Cas9 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate with ab202637 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab202637 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1:  293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate, 10µg (Input).

    Lane 2: ab202637 IP in 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab202637 in 293T cells transfected with Streptococcus thermophilus CRISPR-Cas9-myc whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202637).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded 293T cells transfected blank pcDNA3.1(+)-Myc-His vector with ab202637 at 1/100 dilution. Goat Anti-Rabbit IgG H&L (HRP), ab97051 at 1/500 dillution was used as the secondary antibody. Counterstained with Hematoxylin. Secondary only negative control also shown. Heat mediated antigen retrieval using Tris/EDTA Buffer, pH 9.0 was performed.

    Negative on the 293T cells transfected blank pcDNA3.1(+)-Myc-His vector.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202637). 

  • Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)
    Anti-CRISPR-Cas9 antibody [EPR19619-18] - BSA and Azide free (ab251382)

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