Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)

Key features and details

  • Mouse monoclonal [PCRP-CREB5-1G8] to CREB5 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, WB
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free
    See all CREB5 primary antibodies

  • Description

    Mouse monoclonal [PCRP-CREB5-1G8] to CREB5 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant full length protein corresponding to Human CREB5.
    Database link: Q02930

  • Positive control

    • Flow cyt: HeLa, K562 cells. ICC: K562 cells. WB: HeLa cells.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from bioreactor concentrate

  • Clonality

    Monoclonal

  • Clone number

    PCRP-CREB5-1G8

  • Isotype

    IgG2b

  • Light chain type

    kappa

  • Research areas

Images

  • Immunocytochemistry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Immunocytochemistry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)

    This data was developed using ab277098, the same antibody clone in a different buffer formulation.

    Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 with ab277098 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

  • Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)

    This data was developed using ab277098, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CREB5 using ab277098 at 2 µg/106  cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Western blot - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Anti-CREB5 antibody [PCRP-CREB5-1G8] (ab277098) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cells


    This data was developed using ab277098, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Flow Cytometry - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)

    This data was developed using ab277098, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CREB5 using ab277098 at 2 µg/106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)
    Protein Array - Anti-CREB5 antibody [PCRP-CREB5-1G8] - BSA and Azide free (ab277114)

    This data was developed using ab277098, the same antibody clone in a different buffer formulation.

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277098.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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