Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CPI-17 antibody [E305] (ab32213)

Key features and details

  • Rabbit monoclonal [E305] to CPI-17
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CPI-17 antibody [E305]
    See all CPI-17 primary antibodies

  • Description

    Rabbit monoclonal [E305] to CPI-17

  • Host species

    Rabbit

  • Specificity

    ab32213 recognises CPI-17 and may detect isoform CPI-17 beta.

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human CPI-17 aa 1-100 (N terminal). The exact sequence is proprietary.
    (Peptide available as ab202892)

  • Epitope

    ab32213 reacts with an epitope located in the N terminal region of CPI17 alpha

  • Positive control

    • Hela, Jurkat, MCF-7 and A431 cell lysate

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    E305

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Lanes 1-2 & 4 : purified at 1/1000 dilution
    Lane 3 : RAW264.7 purified at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Anti-CPI-17 antibody [E305] (ab32213) at 1/1000 dilution (purified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-CPI-17 antibody [E305] (ab32213)
    Immunoprecipitation - Anti-CPI-17 antibody [E305] (ab32213)

    ab32213 (purified) at 1/50 immunoprecipitating CPI-17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Anti-CPI-17 antibody [E305] (ab32213) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 17 kDa
    Observed band size: 17 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    Western blot - Anti-CPI-17 antibody [E305] (ab32213)
    All lanes : Anti-CPI-17 antibody [E305] (ab32213) at 1/5000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MCF-7 cell lysate
    Lane 4 : A431 cell lysate

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunoprecipitation - Anti-CPI-17 antibody [E305] (ab32213)
    Immunoprecipitation - Anti-CPI-17 antibody [E305] (ab32213)

    ab32213 (unpurified) at 1/50 immunoprecipitating CPI-17 in MCF7 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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