Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14208(B)] to COX5A - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free
    See all COX5A primary antibodies

  • Description

    Rabbit monoclonal [EPR14208(B)] to COX5A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate; human heart, mouse heart and brain, rat heart and brain tissue lysates. IHC-P: Human colon carcinoma, mouse kidney and rat stomach tissue. IP: Mouse heart tissue lysate.

  • General notes

    ab250141 is the carrier-free version of ab180129. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Western blot - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    All lanes : Anti-COX5A antibody [EPR14208(B)] (ab180129) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Mouse heart lysates
    Lane 5 : Rat brain lysates
    Lane 6 : Rat heart lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    The molecular weight observed is consistent with what has been described in PMID: 28082314.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180129).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling COX5A with Purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180129).

  • Immunoprecipitation - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Immunoprecipitation - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

    Purified ab180129 at 1/80 dilution (2ug) immunoprecipitating COX5A in Mouse heart lysate.

    Lane 1 (input): Mouse heart lysate (10µg)

    Lane 2 (+): ab180129 + Mouse heart lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180129 in Mouse heart lysate.

    Lane 4: Mouse heart lysate captured by ab180129 and detected by anti-Ubiquitin antibody (ab134953).

    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 13 kDa
    The ladder band pattern could be ubiquitylated Cox5a as validated by anti-Ubiquitin antibody (ab134953, Lane4)
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180129).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling COX5A with Purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180129).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling COX5A with Purified ab180129 at 1/3000 dilution (0.5 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180129).

  • Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)
    Anti-COX5A antibody [EPR14208(B)] - BSA and Azide free (ab250141)

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