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Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)

Key features and details

  • Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
  • Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF
  • Reacts with: Cow, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-COX4 + COX4L2 antibody [10G8D12C12]
    See all COX4 + COX4L2 primary antibodies

  • Description

    Mouse monoclonal [10G8D12C12] to COX4 + COX4L2

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-Fr
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    Western blot - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml

    Lane 1 : Isolated mitochondria from Human heart at 5 µg
    Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
    Lane 3 : Isolated mitochondria from HepG2 at 20 µg

    Predicted band size: 20 kDa

  • Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    Flow Cytometry - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
  • Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    Immunocytochemistry/ Immunofluorescence - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
  • Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    Immunohistochemistry (Frozen sections) - Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
    ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.

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