Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261)
Key features and details
- Mouse monoclonal [10G8D12C12] to COX4 + COX4L2
- Suitable for: IHC-Fr, WB, Flow Cyt, ICC/IF
- Reacts with: Cow, Human
- Isotype: IgG2a
Overview
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Product name
Anti-COX4 + COX4L2 antibody [10G8D12C12]
See all COX4 + COX4L2 primary antibodies -
Description
Mouse monoclonal [10G8D12C12] to COX4 + COX4L2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-Fr HumanWB Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Isolated mitochondria from Human heart, Bovine heart and HepG2 cells. Human skeletal muscle (frozen sections). Cultured Human embryonic lung derived fibroblasts (strain MRC5; fixed and permeabilized). Human colon tissue sections. HeLa cells.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab110261 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab110261 was judged as near homogeneity by SDS PAGE. -
Clonality
Monoclonal -
Clone number
10G8D12C12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-COX4 + COX4L2 antibody [10G8D12C12] (ab110261) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from HepG2 at 20 µg
Predicted band size: 20 kDa
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ab110261, at 1 µg/mL, staining COX4 + COX4L2 in HeLa cells by Immunohistochemistry (blue) and an isotype control antibody, at 1 µg/mL, staining of HeLa cells (red).
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ab110261, at 10 µg/ml, staining COX4 + COX4L2 in cultured Human embryonic lung derived fibroblasts by Immunofluorescence. The cells (strain MRC5) were fixed, permeabilized and then labeled with ab110261 followed by an AlexaFluor® 488 conjugated Goat anti-mouse IgG2a isotype specific secondary antibody at 1 µg/ml.
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ab110261, at 1/800, staining COX4 + COX4L2 in skeletal muscle tissue sections from a patient with a single large deletion of the mtDNA by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.
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ab110261, at 1/200, showing COX4 + COX4L2 negative crypts in colon tissue sections from a normal ageing patient by Immunohistochemistry. The tissue was snap frozen and fixed in 4% PFA. Image kindly provided by Dr. J. Murphy and D. Turnbull, Mitochondrial Research Group, Newcastle University.