Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17159] to CFH + CFHL1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CFH + CFHL1 antibody [EPR17159]
    See all CFH + CFHL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17159] to CFH + CFHL1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum, plasma and fetal liver lysates. IHC-P: Human liver and placenta tissues. ICC/IF: HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17159

  • Isotype

    IgG

Images

  • Western blot - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Western blot - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    All lanes : Anti-CFH + CFHL1 antibody [EPR17159] (ab200656) at 1/10000 dilution

    Lane 1 : Human serum
    Lane 2 : Human plasma
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 180,38,44 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Based on the sequence analysis, ab200656 has 100% homology with CFH protein.
    The observed MW is consistent with what has been described in the literature PMID: 19745068.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Cytoplasm staining on Human liver tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Plasma staining on Human placenta tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling CFH + CFHL1 with ab200656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

    Human Lung carcinoma tissue was used as a negative control for CFH + CFHL1.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Immunocytochemistry/ Immunofluorescence - Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling CFH + CFHL1 with ab200656 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). 

    Cytoplasm staining on HepG2 cell line is observed.

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200656 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)
    Anti-CFH + CFHL1 antibody [EPR17159] (ab200656)

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