Anti-COPB2 antibody - C-terminal (ab192924)
Key features and details
- Rabbit polyclonal to COPB2 - C-terminal
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COPB2 antibody - C-terminal
See all COPB2 primary antibodies -
Description
Rabbit polyclonal to COPB2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide within Human COPB2 aa 850 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_004757.1.
Database link: P35606 -
Positive control
- HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192924 was affinity purified using an epitope specific to COPB2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPB2 antibody - C-terminal (ab192924)
Immunohistochemistry of human prostate carcinoma using ab192924 at 1/1000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPB2 antibody - C-terminal (ab192924)Immunohistochemistry of mouse renal cell carcinoma using ab192924 at 1/1000.
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Immunoprecipitation - Anti-COPB2 antibody - C-terminal (ab192924)Detection of COPB2 by Western Blot of Immunprecipitate.
Lane 1: ab192924 at 0.1 µg/ml labeling COPB2 in 293T whole cell lysate (prepared using NETN lysis buffer) immunoprecipitated using ab192924 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG.
Detection: Chemiluminescence with exposure time of 30 seconds. -
Western blot - Anti-COPB2 antibody - C-terminal (ab192924)All lanes : Anti-COPB2 antibody - C-terminal (ab192924) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 30 secondsLysates were prepared using NETN lysis buffer.
