Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)
![Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)](https://www.abcam.com/ps/products/275/ab275890/Images/ab275890-411860-anti-collagen-v-antibody-epr2376254-western-blot-organ.jpg "Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23762-54] to Collagen V - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free
See all Collagen V primary antibodies -
Description
Rabbit monoclonal [EPR23762-54] to Collagen V - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen of this antibody is based on Collagen V-COL5A2, low homology with Collagen V-COL5A1 based on immunogen design.
-
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Rat articular cartilage tissue lysate; Mouse articular cartilage organ culture supernatant.
-
General notes
ab275890 is the carrier-free version of ab275881. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab275890 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23762-54 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Mouse articular cartilage organ culture supernatant at 10 µl
Lane 2 : Mouse articular cartilage organ culture supernatant treated with PNGase F at 10 µl
Lane 3 : Mouse articular cartilage tissue lysate at 20 µg
Lane 4 : Mouse articular cartilage tissue lysate treated with PNGase F at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 32,36 kDa why is the actual band size different from the predicted?This data was developed using ab275881, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20625483).
Glycosylation leads to the shift of the bands.
Exposure time: 6 seconds.
-
![Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)](https://www.abcam.com/ps/products/275/ab275890/Images/ab275890-411859-anti-collagen-v-antibody-epr2376254-western-blot-culture.jpg)
Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Rat placenta organ culture supernatant at 30 µl
Lane 2 : Rat articular cartilage tissue lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 145,32 kDa why is the actual band size different from the predicted?This data was developed using ab275881, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 23262228, 20625483).
Exposure time: Lane 1: 92 seconds Lane 2: 1 seconds.
-
![Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)](https://www.abcam.com/ps/products/275/ab275890/Images/ab275890-411861-anti-collagen-v-antibody-epr2376254-western-blot-tissue.jpg)
Western blot - Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)All lanes : Anti-Collagen V antibody [EPR23762-54] (ab275881) at 1/1000 dilution
Lane 1 : Mouse articular cartilage tissue lysate
Lane 2 : Mouse PBMCs (peripheral blood mononuclear cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 184 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?This data was developed using ab275881, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression pattern is consistent with what has been described in the literature (PMID: 20625483).
Low expression: Mouse PBMCs (database:HPA).
Exposure time: Lane 1: 5 seconds Lane 2: 3 minutes.
-
![Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)](https://www.abcam.com/ps/products/275/ab275890/Images/ab275890-4-benefits-of-recombinant-antibodies.png)
Anti-Collagen V antibody [EPR23762-54] - BSA and Azide free (ab275890)