Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24192-169] to COLEC11 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free
    See all COLEC11 primary antibodies

  • Description

    Rabbit monoclonal [EPR24192-169] to COLEC11 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; Mouse plasma; Human kisney, adrenal gland, liver, skeletal muscle and stomach tissue lysates. IP: Mouse plasma.

  • General notes

    ab278085 is the carrier-free version of ab278081. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278085 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    All lanes : Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human adrenal gland tissue lysate
    Lane 3 : Human liver tissue lysate
    Lane 4 : Human skeletal muscle tissue lysate
    Lane 5 : Human stomach tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28,34 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278081, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:30323815, 28772263, 20956340).

    Low expression:  skeletal muscle, stomach  (PMID:20956340).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 81 seconds.

     

  • Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)

    This data was developed using ab278081, the same antibody clone in a different buffer formulation.

    COLEC11 was immunoprecipitated from 0.35 mg Mouse plasma with ab278081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse plasma 10 μl

    Lane 2: ab278081 IP in Mouse plasma

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278081 in Mouse plasma

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    All lanes : Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Mouse plasma

    Lysates/proteins at 20 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28,34 kDa why is the actual band size different from the predicted?



    This data was developed using ab278081, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:28772263, 30323815).

    Exposure time: 3 minutes.

  • Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)
    Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (ab278085)

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