Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9584] to CMT2 - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CMT2 antibody [EPR9584] - BSA and Azide free
See all CMT2 primary antibodies -
Description
Rabbit monoclonal [EPR9584] to CMT2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248941 is the carrier-free version of ab150363 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as MAD2L1 binding protein
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR9584 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CMT2 antibody [EPR9584] (ab150363) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?This data was developed using ab150363, the same antibody clone in a different buffer formulation.
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This data was developed using ab150363, the same antibody clone in a different buffer formulation.ab150363 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab150363 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)This data was developed using ab150363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CMT2 antibody [EPR9584] - BSA and Azide free (ab248941)This data was developed using ab150363, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue labelling CMT2 binding protein with ab150363 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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