Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17]
See all Cleaved NEAS (Asp1185) primary antibodies -
Description
Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185) -
Host species
Rabbit -
Specificity
Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human Cleaved NEAS (Asp1185) aa 1150-1250. The exact sequence is proprietary.
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Positive control
- HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3830-17 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
Lane 1 : HeLa + Camptothecin cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat + Staurosporine cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 285 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
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