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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Crosslinking

Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)

Price and availability

304 886 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17]
    See all Cleaved NEAS (Asp1185) primary antibodies
  • Description

    Rabbit monoclonal [EPR3830-17] to Cleaved NEAS (Asp1185)
  • Host species

    Rabbit
  • Specificity

    Only detects the large fragment of Alpha Fodrin resulting from cleavage at aspartic acid 1185.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Cleaved NEAS (Asp1185) aa 1150-1250. The exact sequence is proprietary.

  • Positive control

    • HeLa + Camptothecin cell lysate, HeLa cell lysate, Jurkat + Staurosporine cell lysate, Jurkat cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Alpha Fodrin, Cleaved alpha-fodrin (Asp1185)

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3830-17
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Crosslinking
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Images

  • Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
    Western blot - Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
    All lanes : Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)

    Lane 1 : HeLa + Camptothecin cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat + Staurosporine cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 285 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?

  • Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)
    Anti-Cleaved NEAS (Asp1185) antibody [EPR3830-17] (ab92373)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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