Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)
![Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)](https://www.abcam.com/ps/products/250/ab250213/Images/ab250213-405679-anti-cklf-antibody-epr11985-2-bsa-and-azide-free-western-blot.jpg "Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11985(2)] to CKLF - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free
See all CKLF primary antibodies -
Description
Rabbit monoclonal [EPR11985(2)] to CKLF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250213 is the carrier-free version of ab180512. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250213 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11985(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/5000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaThis data was developed using ab180512, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)](https://www.abcam.com/ps/products/250/ab250213/Images/ab250213-402543-anti-cklf-antibody-epr11985-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)All lanes : Anti-CKLF antibody [EPR11985(2)] - C-terminal (ab180512) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : THP-1 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaThis data was developed using ab180512, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)](https://www.abcam.com/ps/products/250/ab250213/Images/ab250213-1-anti-cklf-antibody-epr11985-2-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)This data was developed using ab180512, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CKLF with ab180512 at 1/100 dilution. Anti-rabbit HRP secondary antibody was used and counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)](https://www.abcam.com/ps/products/250/ab250213/Images/ab250213-4-benefits-of-recombinant-antibodies.png)
Anti-CKLF antibody [EPR11985(2)] - BSA and Azide free (ab250213)
