Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CIRP antibody [EPR23628-36] - BSA and Azide free (ab272940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23628-36] to CIRP - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CIRP antibody [EPR23628-36] - BSA and Azide free
    See all CIRP primary antibodies

  • Description

    Rabbit monoclonal [EPR23628-36] to CIRP - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NIH/3T3 and NIH/3T3 treated at 32°C for 48 hours, whole cell lysates; Mouse testis tissue lysate; Rat testis tissue lysate. IP: Mouse testis tissue lysate.

  • General notes

    ab272940 is the carrier-free version of ab246510. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] - BSA and Azide free (ab272940)
    Immunoprecipitation - Anti-CIRP antibody [EPR23628-36] - BSA and Azide free (ab272940)

    CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10ug

    Lane 2: ab246510 IP in Mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 47 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246510).

  • Anti-CIRP antibody [EPR23628-36] - BSA and Azide free (ab272940)
    Anti-CIRP antibody [EPR23628-36] - BSA and Azide free (ab272940)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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