Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKA

Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)

Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12955] to AKAP14 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Human

You may also be interested in

Product image
Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851)
Product image
Recombinant Human AKAP4 protein (ab152924)
Product image
Anti-AKAP1 antibody [EPR10518] - BSA and Azide free (ab249245)
Product image
Anti-AKAP3 antibody (ab254844)

Overview

  • Product name

    Anti-AKAP14 antibody [EPR12955] - BSA and Azide free
    See all AKAP14 primary antibodies
  • Description

    Rabbit monoclonal [EPR12955] to AKAP14 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250165 is the carrier-free version of ab180160.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12955
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA

Images

  • Western blot - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    Western blot - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    All lanes : Anti-AKAP14 antibody [EPR12955] - C-terminal (ab180160) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : NCI-H460 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 23 kDa



    This data was developed using ab180160, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    Immunocytochemistry - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    This data was developed using ab180160, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A549 cells labeling AKAP14 with ab180160 at a 1/100 dilution.
  • Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    Immunoprecipitation - Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)

    This data was developed using ab180160, the same antibody clone in a different buffer formulation.

    Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing AKAP14, using ab180160 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)
    Anti-AKAP14 antibody [EPR12955] - BSA and Azide free (ab250165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-YB1 antibody [EP2708Y] (ab76149)

  •  
  • Product image

    Anti-liver FABP antibody [EPR5895(2)] (ab129203)

  •  
  • Product image

    Anti-LDLC antibody [EPR10896] (ab167416)

  •  
  • Product image

    Anti-Aconitase 2 antibody (ab228923)

  •  
  • Product image

    Anti-Homeobox protein cut-like 1 antibody [CL5275] (ab242194)

  •  
  • Product image

    Anti-hnRNP G antibody (ab244514)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.