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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Glucosaminoglycans

Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)

Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15789] to CHST3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CHST3 antibody [EPR15789] - BSA and Azide free
    See all CHST3 primary antibodies
  • Description

    Rabbit monoclonal [EPR15789] to CHST3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251171 is the carrier-free version of ab193359.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15789
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Glucosaminoglycans
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi

Images

  • Western blot - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    Western blot - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    All lanes : Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab193359, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    Western blot - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    Anti-CHST3 antibody [EPR15789] (ab193359) at 1/1000 dilution + Raw264.7 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa



    This data was developed using ab193359, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    Flow Cytometry - Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    This data was developed using ab193359, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling CHST3 with ab193359 at 1/350 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)
    Anti-CHST3 antibody [EPR15789] - BSA and Azide free (ab251171)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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