Anti-CELF-6 antibody [EPR10155] (ab173282)
![Anti-CELF-6 antibody [EPR10155] (ab173282)](https://www.abcam.com/ps/products/173/ab173282/Images/ab173282-196658-anti-celf-6-antibody-epr10155-flow-cytometry.jpg "Anti-CELF-6 antibody [EPR10155] (ab173282)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10155] to CELF-6
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-CELF-6 antibody [EPR10155]
See all CELF-6 primary antibodies -
Description
Rabbit monoclonal [EPR10155] to CELF-6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
-
Positive control
- Human fetal kidney and fetal brain lysates; HepG2 and 293T cell lysates; Human kidney tissue; 29T cells.
-
General notes
Previously labelled as BRUNOL6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10155 -
Isotype
IgG -
Research areas
Images
-
Flow Cytometry - Anti-CELF-6 antibody [EPR10155] (ab173282)
Flow cytometry analysis of permeabilized 293T cells labeling CELF-6 (red), using ab173282 at a 1/10 dilution, or negative control rabbit IgG (green)
-
![Western blot - Anti-CELF-6 antibody [EPR10155] (ab173282)](https://www.abcam.com/ps/products/173/ab173282/Images/ab173282-196656-anti-celf-6-antibody-epr10155-western-blot.jpg)
Western blot - Anti-CELF-6 antibody [EPR10155] (ab173282)All lanes : Anti-CELF-6 antibody [EPR10155] (ab173282) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal brain lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF-6 antibody [EPR10155] (ab173282)](https://www.abcam.com/ps/products/173/ab173282/Images/ab173282-196657-anti-celf-6-antibody-epr10155-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CELF-6 antibody [EPR10155] (ab173282)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling CELF-6 using ab173282 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-CELF-6 antibody [EPR10155] (ab173282)](https://www.abcam.com/ps/products/173/ab173282/Images/ab173282-4-benefits-of-recombinant-antibodies.png)
Anti-CELF-6 antibody [EPR10155] (ab173282)
