Anti-Cdc20 antibody (ab26483)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc20 antibody
See all Cdc20 primary antibodies -
Description
Rabbit polyclonal to Cdc20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Cdc20.
Read Abcam's proprietary immunogen policy (Peptide available as ab28138.) -
Positive control
- This antibody gave a positive result in the following whole cell lysates: Raji (Human Burkitt's lymphoma cell line) THP1 (Human acute monocytic leukemia cell line) HEK 293 (Human embryonic kidney cell line) HL60 (Human promyelocytic leukemia cell line)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the WD repeat CDC20/Fizzy family.
Contains 7 WD repeats. -
Developmental stage
Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. -
Post-translational
modificationsPhosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. - Information by UniProt
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Database links
- Entrez Gene: 991 Human
- Entrez Gene: 107995 Mouse
- Entrez Gene: 397379 Pig
- Entrez Gene: 64515 Rat
- Omim: 603618 Human
- SwissProt: Q12834 Human
- SwissProt: Q9JJ66 Mouse
- SwissProt: Q5H7C0 Pig
see all -
Alternative names
- bA276H19.3 antibody
- Cdc 20 antibody
- CDC20 antibody
see all
Images
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Western blot - Anti-Cdc20 antibody (ab26483)All lanes : Anti-Cdc20 antibody (ab26483) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)
Lane 2 :THP-1 whole cell lysate (ab7913)
Lane 3 :HEK-293 whole cell lysate (ab7902)
Lane 4 :HL-60 whole cell lysate (ab7914)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (ab26483)ICC/IF image of ab26483 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab26483, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab26483 has been referenced in 16 publications.
- Sun C et al. MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma. Onco Targets Ther 13:10475-10487 (2020). PubMed: 33116627
- Movsisyan N & Pardo LA Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel) 12:N/A (2020). PubMed: 32854244
- Huo X et al. Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data. Sci Rep 9:9924 (2019). PubMed: 31289358
- Li J et al. M2I-1 disrupts the in vivo interaction between CDC20 and MAD2 and increases the sensitivities of cancer cell lines to anti-mitotic drugs via MCL-1s. Cell Div 14:5 (2019). PubMed: 31249607
- Li J et al. The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells. Sci Rep 7:41072 (2017). PubMed: 28112196
Images
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Western blot - Anti-Cdc20 antibody (ab26483)All lanes : Anti-Cdc20 antibody (ab26483) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908)
Lane 2 :THP-1 whole cell lysate (ab7913)
Lane 3 :HEK-293 whole cell lysate (ab7902)
Lane 4 :HL-60 whole cell lysate (ab7914)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands. -
Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (ab26483)
ICC/IF image of ab26483 stained human HEK 293 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab26483, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
