Anti-Cdc20 antibody (ab183479)
Key features and details
- Rabbit polyclonal to Cdc20
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc20 antibody
See all Cdc20 primary antibodies -
Description
Rabbit polyclonal to Cdc20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Cdc20 aa 1-300.
Database link: Q12834 -
Positive control
- NIH 3T3 and INS1 cell lysates; U2OS cells transfected with Cdc20.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cdc20 antibody (ab183479)All lanes : Anti-Cdc20 antibody (ab183479) at 1/2000 dilution
Lane 1 : U2OS cells transfected with control si RNA
Lane 2 : U2OS cells transfected with Cdc20 si RNA
Lane 3 : Rat INS1 cell lysate
Lane 4 : Mouse NIH 3T3 cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 55 kDa
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Immunoprecipitation - Anti-Cdc20 antibody (ab183479)Western blot analysis of Immunoprecipitation using U2OS cells
Lane 1: Input.
Lane 2: control serum.
Lane 3: ab183479.
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Immunocytochemistry/ Immunofluorescence - Anti-Cdc20 antibody (ab183479)Immunofluorescent analysis of U2OS cells showing labeling of Cdc20 with ab183479 at 1/1000 dilution. Anti-Cyclin B labeling is seen in red and DAPI counterstain in blue. Lower image is merged.
