Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)
![Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)](https://www.abcam.com/ps/products/269/ab269718/Images/ab269718-3-59506anticd82antibodybl2flowcytometry.jpg "Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)")
Key features and details
- Mouse monoclonal [B-L2] to CD82 - BSA and Azide free
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD82 antibody [B-L2] - BSA and Azide free
See all CD82 primary antibodies -
Description
Mouse monoclonal [B-L2] to CD82 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD82.
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General notes
ab269718 is a carrier free version of ab47153. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Purification notes
Sterile-filtered through .22 µm and treated to remove endotoxins. Carrier and preservative free. -
Clonality
Monoclonal -
Clone number
B-L2 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-CD82 antibody [B-L2] - BSA and Azide free (ab269718)
A typical staining pattern with the B-L2 monoclonal antibody of lymphocytes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD82 antibody (ab269718)IHC image of ab47153 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47153, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153). -
Immunocytochemistry/ Immunofluorescence - Anti-CD82 antibody (ab269718)ab47153 staining HeLa cells.
ICC/IF was performed with a standard paraformaldehyde technique (non permeabilized, blocked with 0.2% fish gelatin for 30 min at 25°C). Primary antibody used at 1/200 in 0.12% PBS / fish gelatin for one hour. The secondary antibody was a Cy3-conjugated Goat anti-Mouse.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab47153).
