Anti-CD55 antibody [M002] - Extracellular domain (ab253284)
![Anti-CD55 antibody [M002] - Extracellular domain (ab253284)](https://www.abcam.com/ps/products/253/ab253284/Images/ab253284-343976-anticd55antibodym002-westernblot-human.jpg "Anti-CD55 antibody [M002] - Extracellular domain (ab253284)")
Key features and details
- Mouse monoclonal [M002] to CD55 - Extracellular domain
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CD55 antibody [M002] - Extracellular domain
See all CD55 primary antibodies -
Description
Mouse monoclonal [M002] to CD55 - Extracellular domain -
Host species
Mouse -
Specificity
ab253284 detects 60-80 kDa* bands corresponding to the molecular mass of CD55 on SDS-PAGE immunoblots of "native" A431, A549, and MDA-MB-231 cell lysates.
The antibody also detects a "native" recombinant human CD55 extracellular region, but does not detect the denatured form of CD55.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Other Immunogen Type corresponding to Human CD55. Clone M002 was generated from proprietary antigen related to the extracellular region of human CD55 from the MDA-MB-231 breast cancer cell line.
Database link: P08174 -
Positive control
- WB: A549, MDA-MB-231 and A431 cell lysates; human lung tissue lysate (Native). ICC/IF: MDA-MB-231 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
M002 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-CD55 antibody [M002] (ab253284)All lanes : Anti-CD55 antibody [M002] - Extracellular domain (ab253284) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 4 : Human lung tissue lysate
Predicted band size: 41 kDaNative lysates.
Primary for 1 hour at room temperature in PBS/5% non-fat milk/0.045 Tween 20.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [M002] (ab253284)](https://www.abcam.com/ps/products/253/ab253284/Images/ab253284-343977-anticd55antibodym002-imunocytochemistryimmunofluorescence-mdsmb321-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD55 antibody [M002] (ab253284)Paraformaldehyde-fixed MDA-MD-231 (human breast adenocarcinoma cell line) cells stained for CD55 (red) using ab253284 at 1/100 dilution in ICC/IF. The antibody was detected using goat anti-mouse DyLight® 594.