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Anti-CD45 antibody [OX30] (ab6329)

Key features and details

  • Mouse monoclonal [OX30] to CD45
  • Suitable for: Flow Cyt
  • Reacts with: Rat
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CD45 antibody [OX30]
    See all CD45 primary antibodies

  • Description

    Mouse monoclonal [OX30] to CD45

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Rat

  • Immunogen

    Tissue, cells or virus corresponding to Rat CD45. Rat lymph node cells and glycoproteins.

  • Positive control

    • Flow Cyt: Lewis rat splenocytes.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Images

  • Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)
    Flow Cytometry - Anti-CD45 antibody [OX30] (ab6329)

    Lewis rat splenocytes stained with ab6329 (right) or mouse IgG2aκ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6329) or mouse IgG2aκ Isotype (ab18450) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.

    The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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