Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3678(2)] to CD39 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free
    See all CD39 primary antibodies

  • Description

    Rabbit monoclonal [EPR3678(2)] to CD39 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247611 is the carrier-free version of ab108248. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247611 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    Lane 1 : Rat hippocampus tissue lysate
    Lane 2 : Rat pancreas tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70,74 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab108248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    Lane 1 : Mouse placenta tissue lysate
    Lane 2 : Mouse urinary bladder tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab108248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.

  • Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
    Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)

    All lanes : HUVEC whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa
    Observed band size: 70,74 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab108248, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Ref: PMID: 18693757.

  • Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Western blot - Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (unpurified)

    Lane 1 : Human placenta lysate
    Lane 2 : IM-9 cell lysate

    Predicted band size: 58 kDa



    This data was developed using ab108248, the same antibody clone in a different buffer formulation.

     

  • Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)
    Anti-CD39 antibody [EPR3678(2)] - BSA and Azide free (ab247611)

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