Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)
![Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)](https://www.abcam.com/ps/products/280/ab280214/Images/ab280214-1-anti-cd40-antibody-41cd40-western-blot-human-tonsil-lysates10-ug.jpg "Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [41/CD40] to CD40 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CD40 antibody [41/CD40] - BSA and Azide free
See all CD40 primary antibodies -
Description
Mouse monoclonal [41/CD40] to CD40 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human tonsil, lymph node and lymphoma tissue lysates; Raji; Daudi; Ramos whole cell lysates.
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General notes
ab280214 is the carrier-free version of ab280207. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
41/CD40 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)All lanes : Anti-CD40 antibody [41/CD40] (ab280207) at 1/1000 dilution
Lane 1 : Human tonsil lysates
Lane 2 : Human lymph node lysates
Lane 3 : Human lymphoma lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 44,35 kDa why is the actual band size different from the predicted?This data was developed using ab280207, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18312851).
Exposure time: 3 minutes.
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![Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)](https://www.abcam.com/ps/products/280/ab280214/Images/ab280214-2-anti-cd40-antibody-41cd40-western-blot-raji.jpg)
Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)All lanes : Anti-CD40 antibody [41/CD40] (ab280207) at 1/1000 dilution
Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 3 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 44,35 kDa why is the actual band size different from the predicted?This data was developed using ab280207, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat (PMID:10498643).
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18312851).
Exposure time: 3 minutes
