Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [41/CD40] to CD40 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CD40 antibody [41/CD40] - BSA and Azide free
    See all CD40 primary antibodies

  • Description

    Mouse monoclonal [41/CD40] to CD40 - BSA and Azide free

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human tonsil, lymph node and lymphoma tissue lysates; Raji; Daudi; Ramos whole cell lysates.

  • General notes

    ab280214 is the carrier-free version of ab280207. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)
    Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)
    All lanes : Anti-CD40 antibody [41/CD40] (ab280207) at 1/1000 dilution

    Lane 1 : Human tonsil lysates
    Lane 2 : Human lymph node lysates
    Lane 3 : Human lymphoma lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 44,35 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280207, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18312851).

    Exposure time: 3 minutes.

  • Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)
    Western blot - Anti-CD40 antibody [41/CD40] - BSA and Azide free (ab280214)
    All lanes : Anti-CD40 antibody [41/CD40] (ab280207) at 1/1000 dilution

    Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 3 : Ramos (human Burkitt's lymphoma B lymphocyte), whole cell lysate
    Lane 4 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 44,35 kDa why is the actual band size different from the predicted?



    This data was developed using ab280207, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: Jurkat (PMID:10498643).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 18312851).

    Exposure time: 3 minutes

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