Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD39 antibody [EPR22507-48] (ab227840)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22507-48] to CD39
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD39 antibody [EPR22507-48]
    See all CD39 primary antibodies

  • Description

    Rabbit monoclonal [EPR22507-48] to CD39

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta, heart and spleen lysate. IHC-P: Mouse liver and spleen tissue. ICC/IF: Mouse splenocytes. Flow Cyt: Mouse splenocytes. IP: Mouse placenta lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Western blot - Anti-CD39 antibody [EPR22507-48] (ab227840)
    All lanes : Anti-CD39 antibody [EPR22507-48] (ab227840) at 1/1000 dilution

    Lane 1 : Mouse placenta lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 58 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 81 seconds


    The molecular weight observed is consistent with what has been described in the literature (PMID:18036594).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on mouse spleen (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Immunocytochemistry/ Immunofluorescence - Anti-CD39 antibody [EPR22507-48] (ab227840)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse splenocytes labeling CD39 with ab227840 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in subsets of mouse splenocyte. The nuclear stain is DAPI (blue). Counterstained with Anti-CD45R/B220 Antibody (Alexa Fluor® 647)  at a 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD39 antibody [EPR22507-48] (ab227840)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD39 with ab227840 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse liver (PMID: 18567823). The section was incubated with ab227840 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Flow Cytometry - Anti-CD39 antibody [EPR22507-48] (ab227840)

    Flow cytometric analysis of mouse splenocytes labeling CD39 with ab227840 at 1/500. Cells were stained with rabbit IgG Isotype control (ab172730) (Left panel) or ab227840 (Right panel). Then stained with anti-CD45R conjugated to Alexa Fluor® 647. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)
    Immunoprecipitation - Anti-CD39 antibody [EPR22507-48] (ab227840)

    CD39 was immunoprecipitated from 0.35 mg mouse placenta lysate with ab227840 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227840 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse placenta lysate 10 µg (Input).
    Lane 2: ab227840 IP in mouse placenta lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227840 in mouse placenta lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Anti-CD39 antibody [EPR22507-48] (ab227840)
    Anti-CD39 antibody [EPR22507-48] (ab227840)

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