Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)
![Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)](https://www.abcam.com/ps/products/237/ab237972/Images/ab237972-354061-Rat-monoclonal-MEC-147-to-CD34-pbs-only-immunohistochemistry-lung-mouse.jpg "Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)")
Key features and details
- Rat monoclonal [MEC 14.7] to CD34 - BSA and Azide free
- Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD34 antibody [MEC 14.7] - BSA and Azide free
See all CD34 primary antibodies -
Description
Rat monoclonal [MEC 14.7] to CD34 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD34. Specifically, the murine endothelioma cell line tEnd.
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Positive control
- IHC-P: Mouse lung and brain tissue. WB: Mouse lung membrane tissue lysate.
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General notes
ab237972 is a PBS-only buffer format of ab8158.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
MEC 14.7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)
IHC image of CD34 staining in a formalin fixed paraffin embedded mouse lung tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This image was produced using the same antibody clone but in a different formulation; PBS, sodium azide and arginine (ab8158).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)](https://www.abcam.com/ps/products/237/ab237972/Images/ab237972-354063-Rat-monoclonal-MEC-147-to-CD34-pbs-only-immunohistochemistry-brain-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)IHC image of CD34 staining in a formalin fixed paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
This image was produced using the same antibody clone but in a different formulation; PBS, sodium azide and arginine (ab8158).
