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Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)

Key features and details

  • Rat monoclonal [MEC 14.7] to CD34 - BSA and Azide free
  • Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CD34 antibody [MEC 14.7] - BSA and Azide free
    See all CD34 primary antibodies

  • Description

    Rat monoclonal [MEC 14.7] to CD34 - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, IHC-Fr, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD34. Specifically, the murine endothelioma cell line tEnd.

  • Positive control

    • IHC-P: Mouse lung and brain tissue. WB: Mouse lung membrane tissue lysate.

  • General notes

    ab237972 is a PBS-only buffer format of ab8158.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)

    IHC image of CD34 staining in a formalin fixed paraffin embedded mouse lung tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This image was produced using the same antibody clone but in a different formulation; PBS, sodium azide and arginine (ab8158).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [MEC 14.7] - BSA and Azide free (ab237972)

    IHC image of CD34 staining in a formalin fixed paraffin embedded mouse brain tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8158, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This image was produced using the same antibody clone but in a different formulation; PBS, sodium azide and arginine (ab8158).

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