Anti-CD24 antibody [ML5] (ab278509)
![Anti-CD24 antibody [ML5] (ab278509)](https://www.abcam.com/ps/products/278/ab278509/Images/ab278509-1-anti-cd24-antibody-ml5-immunocytochemistry-mcf71.jpg "Anti-CD24 antibody [ML5] (ab278509)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [ML5] to CD24
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-CD24 antibody [ML5]
See all CD24 primary antibodies -
Description
Mouse monoclonal [ML5] to CD24 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
-
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC: MCF7 cells. Flow Cyt: MCF7 cells.
-
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ML5 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Immunocytochemistry - Anti-CD24 antibody [ML5] (ab278509)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 cells labelling CD24 with ab278509 at 1/50 (18.44 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab278509 at a 1/50 dilution followed by ab150080 at a 1/200 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
Confocal image showing membranous staining in MCF7 cell line.
Negative control: MDA-MB-231 (PMID: 18433506).
-
![Flow Cytometry - Anti-CD24 antibody [ML5] (ab278509)](https://www.abcam.com/ps/products/278/ab278509/Images/ab278509-2-anti-cd24-antibody-ml5-flow-cytometry-mdamb2311.jpg)
Flow Cytometry - Anti-CD24 antibody [ML5] (ab278509)Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cells) (Left panel) and MCF7 (Human breast adenocarcinoma epithelial cells) (Right panel) cells labelling CD24 with ab278509 at 1/1000 dilution (0.1µg) (Red) compared with a isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: MDA-MB-231 (PMID: 18433506).
Gated on viable cells.
-
![Anti-CD24 antibody [ML5] (ab278509)](https://www.abcam.com/ps/products/278/ab278509/Images/ab278509-456272-benefitsofrecombinantantibodies.jpg)
Anti-CD24 antibody [ML5] (ab278509)
