Anti-CD18 antibody [C71/16] (ab19580)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [C71/16] to CD18
- Suitable for: Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-CD18 antibody [C71/16]
See all CD18 primary antibodies -
Description
Rat monoclonal [C71/16] to CD18 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse -
Immunogen
Tissue, cells or virus within Mouse CD18. The exact sequence is proprietary.
Sequence:Cell membrane glycoproteins derived from T-cell lymphoma BW5147 cells
Database link: P11835 -
Positive control
- Flow Cyt: Mouse peripheral blood mononuclear cell (PBMC).
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
C71/16 -
Isotype
IgG2a -
Research areas
Images
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Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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