Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µg 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Mouse monoclonal [3D9F1] to CD166
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Human
Isotype: IgG1

Product name

Anti-CD166 antibody [3D9F1]
See all CD166 primary antibodies
Description

Mouse monoclonal [3D9F1] to CD166
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD166 aa 227-381. (Expressed in E.coli).
Database link: Q13740
Positive control
- WB: Human CD166 (aa 227-381) recombinant protein; CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa and K562 cells. IHC-P: Human cervical cancer and bladder cancer tissues.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

3D9F1
Isotype

IgG1
Research areas

Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).
Western blot - Anti-CD166 antibody [3D9F1] (ab233750)

Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution + Human CD166 (aa 227-381) recombinant protein

Predicted band size: 65 kDa

Expected MW is 47 kDa.
Western blot - Anti-CD166 antibody [3D9F1] (ab233750)

All lanes : Anti-CD166 antibody [3D9F1] (ab233750) at 1/500 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD166 (aa 227-381)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 65 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

Paraffin-embedded human cervical cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD166 antibody [3D9F1] (ab233750)

Paraffin-embedded human bladder cancer tissue stained for CD166 using ab233750 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
Flow Cytometry - Anti-CD166 antibody [3D9F1] (ab233750)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD166 with ab233750 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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