Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
![Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)](https://www.abcam.com/ps/products/280/ab280203/Images/ab280203-1-anti-cd161-antibody-epr23831120-immunocytochemistry-human-pbmc.jpg "Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23831-120] to CD161 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CD161 antibody [EPR23831-120] - BSA and Azide free
See all CD161 primary antibodies -
Description
Rabbit monoclonal [EPR23831-120] to CD161 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cytmore details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: Human PBMC cells. Flow Cyt: Human PBMC cells.
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General notes
ab280203 is the carrier-free version of ab259916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280203 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23831-120 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
This data was developed using ab259916, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC cells labelling CD161 with ab259916 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)](https://www.abcam.com/ps/products/280/ab280203/Images/ab280203-2-anti-cd161-antibody-epr23831120-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)This data was developed using ab259916, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)](https://www.abcam.com/ps/products/280/ab280203/Images/ab280203-3-anti-cd161-antibody-epr23831120-flow-cytometry-human-peripheral-blood-mononuclear-cell1.jpg)
Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)This data was developed using ab259916, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.
Gated on viable CD4(-) population.
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![Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)](https://www.abcam.com/ps/products/280/ab280203/Images/ab280203-4-anti-cd161-antibody-epr23831120-flow-cytometry-human-peripheral-blood-mononuclear-cell2.jpg)
Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)This data was developed using ab259916, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.
Gated on viable CD56(-) population.