Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23831-120] to CD161 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CD161 antibody [EPR23831-120] - BSA and Azide free
    See all CD161 primary antibodies

  • Description

    Rabbit monoclonal [EPR23831-120] to CD161 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC: Human PBMC cells. Flow Cyt: Human PBMC cells.

  • General notes

    ab280203 is the carrier-free version of ab259916. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280203 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23831-120

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
    Immunocytochemistry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)

    This data was developed using ab259916, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC cells labelling CD161 with ab259916 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)

    This data was developed using ab259916, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647.

    Gated on viable cells.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)

    This data was developed using ab259916, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.

    Gated on viable CD4(-) population.

  • Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)
    Flow Cytometry - Anti-CD161 antibody [EPR23831-120] - BSA and Azide free (ab280203)

    This data was developed using ab259916, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling CD161 with ab259916 at 1/500 dilution/ Right compared with a Rabbit monoclonal IgG isotype control (ab172730) / Left. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG or ab259916. Then stained with anti-CD4 conjugated to Alexa Fluor® 647 and anti-CD56 conjugated to BV421.

    Gated on viable CD56(-) population.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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