Anti-CCR4 antibody (ab47553)
Key features and details
- Rabbit polyclonal to CCR4
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-CCR4 antibody
See all CCR4 primary antibodies -
Description
Rabbit polyclonal to CCR4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CCR4.
Read Abcam's proprietary immunogen policy (Peptide available as ab47642.) -
Positive control
- This antibody gave a positive signal in the following Mouse lysates: Thymus Tissue, Spleen Tissue and RAW 264.7 Whole Cell.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).Target
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Function
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
Tissue specificity
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsIn natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 12773 Mouse
- SwissProt: P51680 Mouse
- Unigene: 1337 Mouse
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Alternative names
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
Images
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Western blot - Anti-CCR4 antibody (ab47553)All lanes : Anti-CCR4 antibody (ab47553) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
CCR4 contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 55kDa.
Datasheets and documents
References (0)
ab47553 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-CCR4 antibody (ab47553)All lanes : Anti-CCR4 antibody (ab47553) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate
Lane 3 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 32 kDa. We are unsure as to the identity of these extra bands.
CCR4 contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 55kDa.
