Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21168-154] to CCL1 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free
    See all CCL1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21168-154] to CCL1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat treated with 10 ng/ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours. IP: Jurkat treated with 10 ng/ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours.

  • General notes

    ab280356 is the carrier-free version of ab280371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280356 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21168-154

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
    Western blot - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
    All lanes : Anti-CCL1 antibody [EPR21168-154] (ab280356) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
    Lane 2 : Jurkat treated with /ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab280356, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    CCL1 expression is induced by PMA and  anti-CD28 antibody (PMID: 22413885).

    Exposure time: 37 seconds

     

  • Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
    Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)

    This data was developed using ab280356, the same antibody clone in a different buffer formulation.

    CCL1 was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte) treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 µg with ab280356 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 µg

    Lane 2: ab280356 IP in Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280356 in Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 76 seconds

    This blot was developed using a higher sensitivity ECL substrate.

  • Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
    Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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