Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
![Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)](https://www.abcam.com/ps/products/280/ab280371/Images/ab280371-454990-anti-ccl1-antibody-epr21168154-western-blot-t.jpg "Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21168-154] to CCL1 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Overview
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Product name
Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free
See all CCL1 primary antibodies -
Description
Rabbit monoclonal [EPR21168-154] to CCL1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat treated with 10 ng/ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours. IP: Jurkat treated with 10 ng/ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours.
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General notes
ab280356 is the carrier-free version of ab280371. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab280356 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21168-154 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)All lanes : Anti-CCL1 antibody [EPR21168-154] (ab280356) at 1/1000 dilution
Lane 1 : Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate
Lane 2 : Jurkat treated with /ml PMA and 1ug/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This data was developed using ab280356, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
CCL1 expression is induced by PMA and anti-CD28 antibody (PMID: 22413885).
Exposure time: 37 seconds
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![Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)](https://www.abcam.com/ps/products/280/ab280371/Images/ab280371-1-anti-ccl1-antibody-epr21168154-immunoprecipitation-jurkat.jpg)
Immunoprecipitation - Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)This data was developed using ab280356, the same antibody clone in a different buffer formulation.
CCL1 was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte) treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 µg with ab280356 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280356 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours, whole cell lysate 10 µg
Lane 2: ab280356 IP in Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280356 in Jurkat treated with 10ng/ml PMA and 1µg/ml anti-CD28 antibody and 300ng/ml BFA for 21 hours whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds
This blot was developed using a higher sensitivity ECL substrate.
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![Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)](https://www.abcam.com/ps/products/280/ab280371/Images/ab280371-455234-benefits-of-recombinant-antibodies.jpg)
Anti-CCL1 antibody [EPR21168-154] - BSA and Azide free (ab280371)
