Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CaV1.3 antibody [N38/8] (ab84811)

Key features and details

  • Mouse monoclonal [N38/8] to CaV1.3
  • Suitable for: Flow Cyt, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CaV1.3 antibody [N38/8]
    See all CaV1.3 primary antibodies

  • Description

    Mouse monoclonal [N38/8] to CaV1.3

  • Host species

    Mouse

  • Specificity

    No cross-reactivity against Cav1.2

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Mouse
    IHC-P
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Fusion protein corresponding to C terminal amino acids 2025-2161 of Rat Cav1.3

  • Positive control

    • Rat brain lysate IHC-P: Mouse brain tissue.

  • General notes

    At Abcam we are committed to ensuring that our customers have access to the complete dataset available for our products to allow you to select the best product for your research needs. With this in mind, for this product we highlight the fact that it is known by two different clone numbers. It's primary clone number is N38/8, but you may also see it referred to as S38-8 in literature.

    The clone number has been updated from S38-8 to N38/8, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)

    Frozen mouse brain tissue stained for CaV1.3 using ab84811 at 1/1000 dilution in immunohistochemical analysis.

    Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at for 5 minutes at room temperature.

  • Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)
    All lanes : Anti-CaV1.3 antibody [N38/8] (ab84811) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Rat tissue lysate

    Predicted band size: 245 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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