Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)
![Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-3-ab207758277647207758IHC1.jpg "Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20469] to Cathelicidin/CLP - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free
See all Cathelicidin/CLP primary antibodies -
Description
Rabbit monoclonal [EPR20469] to Cathelicidin/CLP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240378 is the carrier-free version of ab207758 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Cathelicidin
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20469 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)
Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-2-ab207758277646207758IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on neutrophils in a blood vessel [PMID: 10858248].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-1-ab207758277645207758IP.jpg)
Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)Cathelicidin/CLP was immunoprecipitated from 0.35 mg of human spleen lysate with ab207758 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207758 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Human spleen lysate, 10 µg (Input).
Lane 2: ab207758 IP in human spleen lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207758 in human spleen lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207758).
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![Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)](https://www.abcam.com/ps/products/240/ab240378/Images/ab240378-4-benefits-of-recombinant-antibodies.png)
Anti-Cathelicidin/CLP antibody [EPR20469] - BSA and Azide free (ab240378)
