Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20469] to Cathelicidin/CLP
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Cathelicidin/CLP antibody [EPR20469]
    See all Cathelicidin/CLP primary antibodies

  • Description

    Rabbit monoclonal [EPR20469] to Cathelicidin/CLP

  • Host species

    Rabbit

  • Specificity

    Specificity for Antibacterial peptide LL-37

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human serum and plasma; Human fetal spleen lysate. IHC-P: Human spleen and tonsil tissues. IP: Human spleen lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Cathelicidin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils of human spleen is observed [PMID: 10858248].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Cathelicidin/CLP with ab207758 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on neutrophils in a blood vessel [PMID: 10858248].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    Western blot - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    All lanes : Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758) at 1/1000 dilution

    Lane 1 : Human serum
    Lane 2 : Human plasma
    Lane 3 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 19 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 seconds; Lane 2: 3 minutes; Lane 3: 2 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID 10858248).

  • Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    Immunoprecipitation - Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)

    Cathelicidin/CLP was immunoprecipitated from 0.35 mg of human spleen lysate with ab207758 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab207758 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human spleen lysate, 10 µg (Input).

    Lane 2: ab207758 IP in human spleen lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207758 in human spleen lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)
    Anti-Cathelicidin/CLP antibody [EPR20469] (ab207758)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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