Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)
![Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)](https://www.abcam.com/ps/products/247/ab247220/Images/ab247220-407767-anti-caspase-9-antibody-e84-bsa-and-azide-free-western-blot.jpg "Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E84] to Caspase-9 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Caspase-9 antibody [E84] - BSA and Azide free
See all Caspase-9 primary antibodies -
Description
Rabbit monoclonal [E84] to Caspase-9 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247220 is the carrier-free version of ab32068 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247220 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E84 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)
This data was developed using ab32068, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (40 µg).
Lane 2: CASP9 knockout HAP1 whole cell lysate (40µg).
Lane 3: HeLa whole cell lysate (40 µg).
Lanes 1 - 3: Merged signal (red and green). Green - ab32068 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32068 was shown to specifically recognize CASP9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CASP9 knockout samples were examined. Wild-type and CASP9 knockout samples were subjected to SDS-PAGE. ab32068 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)](https://www.abcam.com/ps/products/247/ab247220/Images/ab247220-406318-anti-caspase-9-antibody-e84-bsa-and-azide-free-western-blot.png)
Western blot - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)All lanes : Anti-Caspase-9 antibody [E84] (ab32068) at 1/1000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with staurosporine 1uM for 4 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 46 kDa
Additional bands at: 35 kDa, 37 kDa, 46 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesThis data was developed using ab32068, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)](https://www.abcam.com/ps/products/247/ab247220/Images/ab247220-2-anti-caspase-9-antibody-e84-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)This data was developed using ab32068, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-Pro-Caspase-9 RabMAb (ab32068). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Western blot - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)](https://www.abcam.com/ps/products/247/ab247220/Images/ab247220-397856-anti-caspase-9-antibody-e84-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220) -
![Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)](https://www.abcam.com/ps/products/247/ab247220/Images/ab247220-5-benefits-of-recombinant-antibodies.png)
Anti-Caspase-9 antibody [E84] - BSA and Azide free (ab247220)