Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10
  • Suitable for: WB, IHC-P, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Caspase-10/CASP-10 antibody [EPR10890]
    See all Caspase-10/CASP-10 primary antibodies

  • Description

    Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Caspase-10/CASP-10. The exact sequence is proprietary.
    Database link: Q92851

  • Positive control

    • K562 and Jurkat whole cell lysate (ab7899); Human hepatocellular carcinoma tissue; K562 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as Caspase-10.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    All lanes : Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 58 kDa

  • Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

    Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    Flow Cytometry - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

    Flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

  • Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)
    Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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