Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10
Suitable for: WB, IHC-P, IP, Flow Cyt
Reacts with: Human

Product name

Anti-Caspase-10/CASP-10 antibody [EPR10890]
See all Caspase-10/CASP-10 primary antibodies
Description

Rabbit monoclonal [EPR10890] to Caspase-10/CASP-10
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human Caspase-10/CASP-10. The exact sequence is proprietary.
Database link: Q92851
Positive control
- K562 and Jurkat whole cell lysate (ab7899); Human hepatocellular carcinoma tissue; K562 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Previously labelled as Caspase-10.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10890
Isotype

IgG
Research areas

Western blot - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

All lanes : Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 58 kDa
Immunoprecipitation - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Western blot analysis on immunoprecipitation pellet from (1) K562 cell lysate or (2) 1XPBS (negative control) using ab177475 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Caspase-10/CASP-10 with ab177475 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Flow cytometric analysis of permeabilized K562 cells labeling Caspase 10/CASP-10 with ab177475 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
Anti-Caspase-10/CASP-10 antibody [EPR10890] (ab177475)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com