Anti-cardiac Troponin I (phospho T143) antibody (ab58546)
Key features and details
- Rabbit polyclonal to cardiac Troponin I (phospho T143)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-cardiac Troponin I (phospho T143) antibody
See all Cardiac Troponin I primary antibodies -
Description
Rabbit polyclonal to cardiac Troponin I (phospho T143) -
Host species
Rabbit -
Specificity
Detects endogenous levels of cardiac Troponin I only when phosphorylated at threonine 142. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 142 (RPTPLR)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-cardiac Troponin I (phospho T143) antibody (ab58546)All lanes : Anti-cardiac Troponin I (phospho T143) antibody (ab58546) at 1/500 dilution
Lane 1 : Mouse heart extract without immunizing peptide
Lane 2 : Mouse heart extract with immunizing peptide
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
