Anti-CARD9 antibody (ab264412)
Key features and details
- Rabbit polyclonal to CARD9
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CARD9 antibody
See all CARD9 primary antibodies -
Description
Rabbit polyclonal to CARD9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human CARD9 aa 450-500. The exact sequence is proprietary.
Database link: Q9H257 -
Positive control
- WB: SW620, HT-29 and HL-60 whole cell lysates. IP: SW620 whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CARD9 antibody (ab264412)All lanes : Anti-CARD9 antibody (ab264412) at 1/1000 dilution
Lane 1 : SW620 whole cell lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-CARD9 antibody (ab264412)CARD9 was immunoprecipitated from SW620 whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264412 at 100 µl per reaction. Western blot was performed from the immunoprecipitate using ab264412 at 1/1000 dilution.
Lane 1: ab264412 IP in SW620 whole cell lysate.
Lane 2: Control IgG IP in SW620 whole cell lysate.Detection: Chemiluminescence with an exposure time of 10 seconds.
Lysates prepared using NETN lysis buffer.
