Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)]
    See all Carboxypeptidase A1+A2+B primary antibodies

  • Description

    Rabbit monoclonal [EPR12087(B)] to Carboxypeptidase A1+A2+B

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human, mouse and rat pancreas lysates; Human pancreas tissue; BxPC3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/5000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Immunocytochemistry/ Immunofluorescence - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)

    Immunofluorescent analysis of BxPC3 cells (paraformaldehyde-fixed, 4%) labeling Carboxypeptidase A with ab181146 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Western blot - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    All lanes : Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146) at 1/1000 dilution

    Lane 1 : Rat pancreas lysate
    Lane 2 : Mouse pancreas lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Carboxypeptidase A with ab181146 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)
    Anti-Carboxypeptidase A1+A2+B antibody [EPR12087(B)] (ab181146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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