Anti-carabin antibody (ab77625)
Key features and details
- Goat polyclonal to carabin
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-carabin antibody
See all carabin primary antibodies -
Description
Goat polyclonal to carabin -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Horse, Dog, Rhesus monkey
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Immunogen
Synthetic peptide:
C-PRRKPQTRGKTFH
, corresponding to internal sequence amino acids 405-417 of Human carabin (NP_940919.1). -
Positive control
- Human spleen lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab77625 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-carabin antibody (ab77625)
Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77625 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western blot - Anti-carabin antibody (ab77625)All lanes : Anti-carabin antibody (ab77625) at 2 µg/ml
Lane 1 : Human Spleen
Lane 2 : Mouse thymus
Lysates/proteins at 35 µg per lane.
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Western blot - Anti-carabin antibody (ab77625)Lanes 1 & 3 : Anti-carabin antibody (ab77625) at 0.5 µg/ml
Lane 2 : Anti-carabin antibody (ab77625) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : PBM cell lysates
Lane 3 : A431 cell lysate (negative control)
Lysates/proteins at 35 µg per lane.
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Western blot - Anti-carabin antibody (ab77625)Anti-carabin antibody (ab77625) at 2 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
