Anti-Caprin-1 antibody (ab205377)
Key features and details
- Goat polyclonal to Caprin-1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Caprin-1 antibody
See all Caprin-1 primary antibodies -
Description
Goat polyclonal to Caprin-1 -
Host species
Goat -
Specificity
ab205377 is expected to recognize both reported isoforms (NP_005889.3; NP_976240.1). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Caprin-1 aa 450-550 (internal sequence) (Cysteine residue).
Database link: Q14444 -
Positive control
- HeLa and jurkat cell lysates.
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General notes
Previously labelled as GPIP137.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab205377 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caprin-1 antibody (ab205377)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human cortex staining Caprin-1 with ab205377 at 5 μg/ml
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Western blot - Anti-Caprin-1 antibody (ab205377)All lanes : Anti-Caprin-1 antibody (ab205377) at 0.3 µg/ml
Lane 1 : Jurkat cell lysate (in RIPA buffer)
Lane 2 : HeLa cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 78 kDa
Observed band size: 140,80 kDa why is the actual band size different from the predicted?The ~140 kDa band corresponds to the reported p137 protein (Ellis and Luzio, J Biol Chem. 1995 Sep 1;270(35):20717-23.PMID: 7657653).
