Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP8471] to cAMP - BSA and Azide free
  • Suitable for: ICC/IF, ELISA
  • Reacts with: Species independent

Overview

  • Product name

    Anti-cAMP antibody [EP8471] - BSA and Azide free
    See all cAMP primary antibodies

  • Description

    Rabbit monoclonal [EP8471] to cAMP - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody does not cross react with cGMP, GMP, GDP, cIMP, cCMP, or adenosine.

  • Tested applications

    Suitable for: ICC/IF, ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to cAMP conjugated to keyhole limpet haemocyanin.

  • General notes

    ab179459 is the carrier-free version of ab134901.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP8471

  • Isotype

    IgG

  • Research areas

Images

  • ELISA - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    ELISA - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    Competitive ELISA assay using ab134901. 50 µl cAMP-SPDP-BSA was coated in 96-wells. After 1% BSA blocking, serial dilution of ab134901, 25 µl of cAMP, and H2O (negative control) were added. HRP conjugated goat anti-rabbit IgG-Fc antibody was used to develop the color.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134901).

  • Immunocytochemistry/ Immunofluorescence - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    Immunocytochemistry/ Immunofluorescence - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

    ab134901 staining cAMP in SK-N-SH cells treated with (R)-(+)-Methanandamide (ethanol solution) (ab120361), by ICC/IF. Increase in cAMP expression correlates with increased concentration of (R)-(+)-Methanandamide (ethanol solution), as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120361 ((R)-(+)-Methanandamide (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab134901 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134901).

  • Immunocytochemistry/ Immunofluorescence - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    Immunocytochemistry/ Immunofluorescence - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

    This ICC/IF data was generated using the same anti-cAMP antibody clone, EP8471, in a different buffer formulation (cat# ab134901).

    ab134901 staining cAMP in SK-N-SH cells treated with (R)-(+)-Methanandamide (ethanol solution) (ab120361), by ICC/IF. Increase in cAMP expression correlates with increased concentration of (R)-(+)-Methanandamide (ethanol solution), as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120361 ((R)-(+)-Methanandamide (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab134901 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

  • ELISA - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    ELISA - Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

    This ELISA data was generated using the same anti-cAMP antibody clone, EP8471, in a different buffer formulation (cat# ab134901).

    Competitive inhibition ELISA using ab134901. 50µl of cAMP-SPDP-BSA was coated in 96-wells. After 1% BSA blocking, 25 µl cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP, Adenosine, H2O and 25 µl of ab134901 were added. HRP conjugated goat anti-rabbit IgG-Fc-HR antibody was used to develop the color.

  • Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)
    Anti-cAMP antibody [EP8471] - BSA and Azide free (ab179459)

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