Anti-CamKII gamma antibody [8G10C1] (ab201966)
Key features and details
- Mouse monoclonal [8G10C1] to CamKII gamma
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CamKII gamma antibody [8G10C1]
See all CamKII gamma primary antibodies -
Description
Mouse monoclonal [8G10C1] to CamKII gamma -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB RatHuman -
Immunogen
Recombinant fragment corresponding to Human CamKII gamma aa 300-500. Expressed in E. Coli.
Database link: Q13555-1 -
Positive control
- IHC-P: Human prostate cancer and bladder cancer tissues. ICC/IF: Jurkat cells. WB: PC12, Jurkat, T47D, HeLa and HepG2 cell lysates; Human recombinant CamKII gamma (aa322-481) protein; Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR280299-5 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8G10C1 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAMK2G knockout HeLa cell lysate
Lane 3 : T-47D cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaLanes 1-3: Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.
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Flow cytometric analysis of Jurkat cells labeling CamKII gamma with ab201966 at 1/200 dilution (green). Negative control (red).
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All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : PC12 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : T47D cell lysate
Lane 4 : HepG2 cell lysate
Predicted band size: 63 kDa
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All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate
Predicted band size: 63 kDa
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Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution + Human recombinant CamKII gamma (aa322-481) protein
Predicted band size: 63 kDa(Expected MW is 44 kDa)