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Anti-CamKII gamma antibody [8G10C1] (ab201966)

Price and availability

274 732 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-CamKII gamma antibody [8G10C1] (ab201966)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8G10C1] to CamKII gamma
  • Suitable for: IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CamKII gamma antibody [8G10C1]
    See all CamKII gamma primary antibodies
  • Description

    Mouse monoclonal [8G10C1] to CamKII gamma
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human CamKII gamma aa 300-500. Expressed in E. Coli.
    Database link: Q13555-1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human prostate cancer and bladder cancer tissues. ICC/IF: Jurkat cells. WB: PC12, Jurkat, T47D, HeLa and HepG2 cell lysates; Human recombinant CamKII gamma (aa322-481) protein; Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR280299-5 are from Tissue Culture Supernatant

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8G10C1
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calmodulin / CaMK
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Images

  • Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CAMK2G knockout HeLa cell lysate
    Lane 3 : T-47D cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 63 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab201966 observed at 63 kDa. Red - loading control, ab181602 observed at 37 kDa.

    ab201966 Anti-CamKII gamma antibody [8G10C1] was shown to specifically react with CamKII gamma in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264712 (knockout cell lysate ab258340) was used. Wild-type and CamKII gamma knockout samples were subjected to SDS-PAGE. ab201966 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (ab201966)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CamKII gamma antibody [8G10C1] (ab201966)

    Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CamKII gamma with ab201966 at 1/200 dilution followed by DAB staining. 

  • Flow Cytometry - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Flow Cytometry - Anti-CamKII gamma antibody [8G10C1] (ab201966)

    Flow cytometric analysis of Jurkat cells labeling CamKII gamma with ab201966 at 1/200 dilution (green). Negative control (red).

  • Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : T47D cell lysate
    Lane 4 : HepG2 cell lysate

    Predicted band size: 63 kDa

  • Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    All lanes : Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Human recombinant CamKII gamma (aa322-481)-hIgGFc transfected HEK293 cell lysate

    Predicted band size: 63 kDa

  • Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Western blot - Anti-CamKII gamma antibody [8G10C1] (ab201966)
    Anti-CamKII gamma antibody [8G10C1] (ab201966) at 1/500 dilution + Human recombinant CamKII gamma (aa322-481) protein

    Predicted band size: 63 kDa



    (Expected MW is 44 kDa)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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