Anti-Calponin 3 antibody - C-terminal (ab204365)
Key features and details
- Rabbit polyclonal to Calponin 3 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calponin 3 antibody - C-terminal
See all Calponin 3 primary antibodies -
Description
Rabbit polyclonal to Calponin 3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to Human Calponin 3 aa 250 to the C-terminus (C terminal).
Database link: Q15417 -
Positive control
- WB: RT-4 & U-251 MG whole cell lysates; IHC-P: Human placenta, kidney and testis tissue. ICC: U-2 OS whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Calponin 3 antibody - C-terminal (ab204365)All lanes : Anti-Calponin 3 antibody - C-terminal (ab204365) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate
Predicted band size: 36 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (ab204365)Immunohistochemical analysis of human placenta tissue labeling Calponin 3 in epithelial cells with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-Calponin 3 antibody - C-terminal (ab204365)Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling Calponin 3 in the cytosol and actin filaments with ab204365 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (ab204365)Immunohistochemical analysis of human kidney tissue labeling Calponin 3 in membrane and cytoplasm of cells in tubules with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (ab204365)Immunohistochemical analysis of human testis tissue labeling Calponin 3 in peritubular myoid cells with ab204365 at a 1/1000 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 3 antibody - C-terminal (ab204365)Immunohistochemical analysis of human skeletal muscle tissue labeling Calponin 3 in myocytes with ab204365 at a 1/1000 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
